Part:BBa_K2960004

The mlrD transporter protein

A cassette of enzymes endogenous to Sphingopyxis sp. has been shown to sequentially break down microcystin-LR (the most common and toxic microcystin that results from harmful algal blooms). The enzymes have been named mlrA, mlrB, mlrC, mlrD, mlrE, and mlrF.

This is a composite part consisting of a constitutively-active promoter sequence (J23100), a ribosome binding site (BBa_J61100), the mlrD gene (BBa_K2960009), a 3x FLAG tag (BBa_K823034), and a terminator (BBa_B0015). MlrD is a transporter protein in the microcystin degradation pathway. It transports microcystins into the bacterial cell.

The 3X FLAG tag at the end of the sequence permits protein expression to be detected, quantified and/or purified by western blot, SDS-PAGE, and other methods.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 173
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 357
  
• 1000
  COMPATIBLE WITH RFC[1000]